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antipgam1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antipgam1
    Antipgam1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antipgam1/PGAM1+Rabbit+mAb/pm41637534-381-103-106
    Average 94 stars, based on 32 article reviews
    antipgam1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: NFS1 Regulates IDH2 to Attenuate Abdominal Aortic Aneurysms via Interacting With SP2.
    Article Snippet: The protein samples were boiled for 10 min, separated using 10%SDS-PAGE (M00656, GenScript, NJ, USA) and transferred to polyvinylidene fluoride membranes. .. For immunoblotting, the membranes were blocked in blocking buffer (P30500, NCM Biotech, Suzhou, China) for 40 min at 25 °C, and the proteins were detected after incubating the membranes with primary antibodies overnight at 4 °C as follows: anti-αSMA (1:1000, ab184705, Abcam, Cambridge, MA, USA), anti-OPN (1:1000, 22952-1-AP, Proteintech, Wuhan, China), anti-NFS1 (1:1000, 67021-1-lg, Proteintech, Wuhan, China), anti-CSE (1:1000, 602341-1-lg, Proteintech, Wuhan, China), anti-Cyclin d1 (1:1000, A11022, Abclonal, Wuhan, China), anti-PCNA (1:1000, A12027, Abclonal, Wuhan, China), anti-IDH2 (1:1000, A7190, Abclonal, Wuhan, China), anti-PFKP (1:1000, 8164t, CST, Danvers, MA, USA), anti-HK2 (1:1000, 2867t, CST, Danvers, MA, USA), anti-ENO1 (1:1000, 3810t, CST, Danvers, MA, USA), antiPGAM1 (1:1000, 12098s, CST, Danvers, MA, USA), anti-LDHA (1:1000, 3582t, CST, Danvers, MA, USA), anti-LDHB (1:1000, 56298s, CST, Danvers, MA, USA), anti-ALDOA (1:1000, 8060s, CST, Danvers, MA, USA), anti-PKM2 (1:1000, 4053s, CST, Danvers, MA, USA), anti-HA-Tag (1:1000, 3724t, CST, Danvers,MA, USA), anti-SP1 (1:1000, 21962-1-ap, Proteintech, Wuhan, China), anti-SP2 (1:1000, 25000-1-ap, Proteintech,Wuhan, China), anti-SP5 (1:1000, 10774-1-ap, Proteintech, Wuhan, China), anti-ZNF532 (1:1000, A305-442A, Bethyl Laboratories, Montgomery, TX, USA). .. The internal controls for normalizing protein abundance were anti-α-tubulin (1:5000, 11224-1-AP, Proteintech, Wuhan, China), anti-β-actin (1:10 000, ab8226, Abcam, Cambridge, MA, USA), anti-COX IV (1:1000, 11242-1- ap, Proteintech, Wuhan, China), anti-VCAM1 (1:1000, ab174279, Abcam, Cambridge, MA, USA), and anti-ICAM1 (1:1000, ab222736, Abcam, Cambridge, MA, USA).

    Blocking Assay:

    Article Title: NFS1 Regulates IDH2 to Attenuate Abdominal Aortic Aneurysms via Interacting With SP2.
    Article Snippet: The protein samples were boiled for 10 min, separated using 10%SDS-PAGE (M00656, GenScript, NJ, USA) and transferred to polyvinylidene fluoride membranes. .. For immunoblotting, the membranes were blocked in blocking buffer (P30500, NCM Biotech, Suzhou, China) for 40 min at 25 °C, and the proteins were detected after incubating the membranes with primary antibodies overnight at 4 °C as follows: anti-αSMA (1:1000, ab184705, Abcam, Cambridge, MA, USA), anti-OPN (1:1000, 22952-1-AP, Proteintech, Wuhan, China), anti-NFS1 (1:1000, 67021-1-lg, Proteintech, Wuhan, China), anti-CSE (1:1000, 602341-1-lg, Proteintech, Wuhan, China), anti-Cyclin d1 (1:1000, A11022, Abclonal, Wuhan, China), anti-PCNA (1:1000, A12027, Abclonal, Wuhan, China), anti-IDH2 (1:1000, A7190, Abclonal, Wuhan, China), anti-PFKP (1:1000, 8164t, CST, Danvers, MA, USA), anti-HK2 (1:1000, 2867t, CST, Danvers, MA, USA), anti-ENO1 (1:1000, 3810t, CST, Danvers, MA, USA), antiPGAM1 (1:1000, 12098s, CST, Danvers, MA, USA), anti-LDHA (1:1000, 3582t, CST, Danvers, MA, USA), anti-LDHB (1:1000, 56298s, CST, Danvers, MA, USA), anti-ALDOA (1:1000, 8060s, CST, Danvers, MA, USA), anti-PKM2 (1:1000, 4053s, CST, Danvers, MA, USA), anti-HA-Tag (1:1000, 3724t, CST, Danvers,MA, USA), anti-SP1 (1:1000, 21962-1-ap, Proteintech, Wuhan, China), anti-SP2 (1:1000, 25000-1-ap, Proteintech,Wuhan, China), anti-SP5 (1:1000, 10774-1-ap, Proteintech, Wuhan, China), anti-ZNF532 (1:1000, A305-442A, Bethyl Laboratories, Montgomery, TX, USA). .. The internal controls for normalizing protein abundance were anti-α-tubulin (1:5000, 11224-1-AP, Proteintech, Wuhan, China), anti-β-actin (1:10 000, ab8226, Abcam, Cambridge, MA, USA), anti-COX IV (1:1000, 11242-1- ap, Proteintech, Wuhan, China), anti-VCAM1 (1:1000, ab174279, Abcam, Cambridge, MA, USA), and anti-ICAM1 (1:1000, ab222736, Abcam, Cambridge, MA, USA).



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